Isozyme data were used to assess genetic diversity within and among a subdivided population of the salt-tolerant Batini barley landrace. Population diversity and its components were esti- mated on the basis of 12 isozymes scored on 450 single plants representing seven subpopu- lations. Two principal components, based on mean gene diversity, Shannon’s diversity index, percentage polymorphic loci, genetic identity and genetic distance among subpopu- lations accounted for 91.7% of total variation and separated the subpopulations into four dis- tinct groups. Contributions to total diversity by individual subpopulations were partitioned into intra- and inter-population components. The level of population differentiation indicates that a large percentage of total genetic diversity was apportioned within subpopulations. The pre- sence of valuable genetic diversity in this landrace was confirmed.